MICROSCOPES

  • THOR - Nikon N-SIM structured illumination super-resolution and A1 laser scanning confocal microscope
    Number of Lasers 6
    Laser Lines & Filters Lasers: 405, 445, 488, 515, 561, 647 (nm)

    Filters: DAPI, GFP, RFP, Brightfield

    Objectives Standard objectives:
    • 4x/0.2NA Plan APO λ WD 20 (mm)
    • 10x/0.45NA Plan Apo λ WD 4 (mm)
    • 20x/0.75NA Plan Apo VC λ WD 1 (mm)
    • 40x/0.6NA S Plan Fluor ELWD 3.6-2.8 (mm)
    • 40x/0.95NA Plan Apo λ WD 0.25-0.17 (mm)
    • 100x/1.49NA Oil SR Apo TIRF WD 0.12 (mm)
    Sensors Andor iXon X3 EM-CCD (SIM) 512 X 512 16 bit and 2x GaAsP, 2x PMT (A1 confocal)
    Software NIS Elements
    Features and Capabilities Details: Nikon N-SIM super-resolution; A1 laser scanning confocal with high sensitivity GaAsP detectors; SOLA Light engine; Motorized X, Y, Z; Nikon Perfect Focus System; DIC at 10x, 20x, 40x, 60x, and 100x; TOKAI HIT Cell Incubator 1 x 35 (mm) dish only (option).
     
  • Thor User Guide - Available to current users only (by request)

    Nikon N-SIM sample prep guidelines

    Thor2

    Thor1

     

  • Yokogawa CellVoyager™  CV1000 Confocal Scanner System (Distributed by Olympus)
    Number of Lasers 3
    Laser Lines Laser: 405, 488, 561 (nm) & Brightfield
    Objectives Standard objectives:
    • 10x/0.4NA UPLSAPO WD 3.1 (mm)
    • 20x/0.75NA UPLSAPO WD 0.65 (mm)
    • 20X/0.45NA LUCPLFLN WD 6.4-7.6 (mm)
    • 40x/0.6NA LUCPLFLN WD 2.7-4.0 (mm)
    • 40x/1.30NA UPLFLN OIL LWD 0.2 (mm)
    • 60x/1.35NA UPLSAPO OIL WD 0.15 (mm)

    Objectives available upon request:

    • 4x/0.13NA UPlanFl N UIS2 WD 17 (mm)
    • 60x/1.2NA UPLSAPO WATER WD 0.28
    • 100x/1.40NA UPLSAPO OIL WD 0.13
    Sensors Hamamatsu Photonics | ImagEM X2 EM-CCD Camera C9100-14 High Resolution Format With 1024 x 1024 Pixels
    Software

    CV1000 Acquisition Software 1.06.06

    CV1000 Viewer Software 1.06.06 Download Link

    Features and Capabilities Details: Equipped with an ultra-sensitive camera & microlens-enhanced dual nipkow scanning disk (disks 50 μm, 25 μm & disk removed for widefield) ideal for low phototoxicity/photo bleaching. Ideal scope for live cell incubation at high temporal resolution, multi-point time-lapse imaging and multi day time-lapse. With the added benefit of brightfield montaging & low-medium throughput screening.


    Standard sample holders:

    • Standard slides
    • Multiwell slides
    • Multiwell plate
    • The above are all temperature, CO2 and humidity controlled chambers

    Environmentally controlled chambers:

    • Single 35 (mm) dish
    • Triple 35 (mm) dish
    • Multiwell plate

     

  • IRON MAN - Zeiss 510 Confocal Laser Scanning Microscope
    Number of Lasers 4
    Laser Lines Lasers: 405, 458, 477, 488, 514, 543, 633 (nm)

    Filters: DAPI, GFP, RFP, Brightfield

    Objectives Standard objectives:
    • 5x/0.15NA EC Plan-Neofluar WD 3.1 (mm)
    • 10x/0.30NA EC Plan-Neofluar WD 4.0 (mm)
    • 20X/0.80NA Plan Apochromat WD 0.5 (mm)
    • 40x/1.30NA EC Plan-Neofluar Oil WD 0.21 (mm)
    • 63x/1.40NA Plan Apochromat Oil WD 0.19 (mm)
    • 100x/1.40NA Plan Apochromat Oil WD 0.17 (mm)
    Sensors 3x PMT
    Software Zeiss Zen 2009
    Features and Capabilities Details: FRET; FRAP; Z-Drive
  • Iron Man User Guide (v1.4, 11/2016)

    Zen 2009 User Guide

    Zen 2009 Quick Start Guide

     

    • BLACK WIDOW - Nikon Inverted Spinning Disk Confocal Microscope
      Number of Lasers 4
      Laser Lines 405, 488, 561, 647 (nm)
      Objectives Standard objectives:
      • 10x/0.45NA Plan Apo λ WD 4 (mm)
      • 20x/0.75NA Plan Apo VC λ WD 1 (mm)
      • 40x/0.95NA Plan Apo λ WD 0.25-0.17 (mm)
      • 100x/1.45NA Plan Apo λ Oil WD 0.13 (mm)
      Sensor

      Hamamatsu ImagEM EM-CCD (widefield) ; Andor iXon Ultra 897 EM-CCD

      Both cameras are equipped with 512 X 512 16 bit imaging array

      Software NIS Elements
      Features and Capabilities Details: Fast Acquisition; single point FRAP/PA with 405 nm Coherent OBIS laser; SOLA Light engine; Motorized X, Y, Z; Nikon Perfect Focus System; TOKAI HIT Cell Incubator (option); 1 X 35 (mm) dish only. Capable of multipoint montaging
    • Black Widow User Guide (v.1.0, 06/2015)

      BlackWidow

    • DANTE (Orth Lab) - Olympus IX81 Inverted Widefield Microscope
      Light Source Prior Lumen 200
      Filters Standard filter configurations:
      • DAPI, Hoechst
      • CFP, cerulean
      • Alexa488, FITC, GFP
      • YFP, Venus
      • Alexa555, 568, 594, TxRed, mRFP, mCherry
      • Alexa647, Cy5
      • Brightfield & Phase-contrast (20x, 40x objectives)

      (These are common molecules imaged, for excitation and emission wavelengths. For further inquiry please see the user manual.)

      Objectives Standard objectives:
      • 4x/0.13NA UPlanFl N UIS2 WD 17 (mm)
      • 10x/0.40NA UPlanApo Ph1 WD 3.1 (mm)
      • 20X/0.70NA UPlanApo Ph2 WD 0.65 (mm)
      • 40x/0.75NA UPlanFl N Ph2 WD 0.51 (mm)
      • 100x/1.40NA UPlanSapo Oil WD 0.13 (mm)
      Sensor Hamamatsu Orca R2 CCD
      Software Slidebook 6
      Features and Capabilities Details: Multi-point live cell imaging with InVivo Sci. humidified chamber; 1x35mm, multi-well plates, pre-mixed 5% CO2, balance air (please contact Dr. Orth); Phase; Prior Scientific X,Y,Z-Drive, FRET (CFP --> YFP), GFP/mCherry Dual Dichroic
    • Dante User Guide (v1.2, 11/2014)

      --> If you’re interested in performing long-term time-lapse imaging, please contact Dr. Orth prior to starting.

  • Pepper- Nikon Inverted Widefield Microscope
    Light Source X - Cite 120
    Filters Standard filter configurations:
    • DAPI, Hoechst
    • Alexa488, FITC, GFP
    • TRITC, Alexa555, 568, 594, mRFP, mCherry
    • Alexa647, Cy5
    Objectives Standard objectives:
    • 10x/0.45NA Plan APO λ WD 4 (mm)
      • DIC N1
    • 40x/0.95NA Plan Apo λ WD 0.14 (mm)
      • DIC M/N2
    • 60x/1.40NA Plan Apo OIL VC λ WD 0.13 (mm)
      • DIC N2
    Sensors CoolSNAP HQ2 Monochrome Camera; 16 bit
    Software MetaMorph 7.7.0.0
    Features and Capabilities Details: ASI MS-2000 XYZ, DIC 10x, 40x, 60x, Brightfield
  • Pepper's user guide is coming soon!

     

     

  • LOKI - Nikon E600 Upright Widefield Microscope
    Light Source Brightfield
    Objectives Standard objectives:
    • 10x/0.3NA S Plan Fluor Ph1 DLL WD 16.0 (mm)
    • 20x/0.5NA S Plan Fluor Ph1 DLL WD 2.1 (mm)
    • 40x/0.75NA Plan Fluor Ph2 DLL WD 0.72 (mm)
    • 100x/1.30NA Plan Fluor Oil Ph3 DLL WD 0.2 (mm)
    Sensors Nikon Digital Sight DS-Qi, Spot Insight
    Software NIS Elements, Spot 5.1 Basic
    Features and Capabilities Details: DIC at 40x; Phase; Color Imaging (great for histology samples); Prior OptiScan Z-Drive

    Loki User Guide (v1.2, 03/2015)

    • CAPTAIN AMERICA - Leica DMRXA Upright Widefield Microscope
      Light Source Sutter Lambda LS
      Filters Standard filter configurations:
      • DAPI, Hoechst
      • Alexa488, FITC, GFP
      • TRITC, Alexa555, 568, Cy3
      • Alexa647, Cy5
      Objectives Standard objectives:
      • 10x/0.3NA HC PL FLUOTAR WD ?
      • 20x/0.7NA Plan APO WD ?
      • 40x/1.25-0.75NA HCX PL APO
      • 63x/1.4-0.6NA HCX PL APO Oil
      • 100x/1.4-0.7NA PL APO Oil
      Sensors QImaging Retiga 2000R CCD camera
      Software Slidebook 6
      Features and Capabilities Details: Motorized X,Y, Z; rapid 4 color imaging with Sedat quad set

      Captain America User Guide (v.1.0, 08/2016)