Department of MCD Biology, Campus Box 347, University of Colorado, Boulder, CO 80309, BoulderEM@colorado.edu

Equipment and Rates

Equipment Prices per hour unless noted CU System External Non-Profit Industry
FEI Tecnai F30, 300kV FEG-TEM $85 $175 $350
FEI Tecani F20, 200kV FEG-TEM $75 $160 $330
FEI Tecnai T12 Spirit, 120kV LaB6 filament TEM $64 $126 $144
FEI Philips CM100 $64 $126 $144
FEI Philips CM10 $64 $126 $144
Unchained Labs pUNk DLS $30 $50 $60
Nikon 80i with Instec cryo-stage $18 $31 $36
Wohlwend Compact 02 High Pressure Freezer $7/shot $12/shot $14/shot
Leica EM BAF060 Freeze-etch System $30/run $52/run $60/run
FEI Vitrobot Mark IV $18 $31 $36
Manual plunge freeze device $18 $31 $36
Automatic Freeze-Substitution Systems (AFS) $60/run $105/run $120/run
Leica Ultracut UCT (plastic) $18 $31 $36
Leica Ultracut UCT (w/cryo attachment) $18 $31 $36
Leica UC6 $18 $31 $36
Computer Work Stations $9 $15 $18
GPU Work Station $15 $22 $30
Technical Assistance and Training $60 $104 $120

Facility users who wish to inquire about rates please contact BoulderEM@colorado.edu

Equipment

Microscopes for Electron Microscopy and Cryo-EM

Technai F20

FEI Tecnai F30, 300kV FEG-TEM

  • Equipped with a Gatan Ultrascan US-4000 (4k x 4k) CCD
  • Soon to be equipped with a Gatan K2 Summit (4k x 4k) direct detector, and a Gatan OneView (4k x 4k) CCD
  • Cameras are typically operated with binning to 2K x 2K to maximize efficiency and throughput
  • Typical uses are plastic section tomography and cryo-microscopy and cryo-tomography on thicker samples

Technai F30

FEI Tecani F20, 200kV FEG-TEM

  • Equipped with FEG, Gatan Ultrascan US-4000 4K x 4K camera; usually operated with binning to 2K x 2K for greater efficiency.
  • Typical uses are plastic section and cryo-tomography on thinner samples, examination of cryo-samples, and acquisition for single-particle reconstruction

Conventional TEMs

Technai Spirit

FEI Tecnai T12 Spirit, 120kV LaB6 filament TEM

  • Equipped with a side-mount AMT (2k x 2k) CCD for fast scanning, and an FEI Eagle (4k x 4k) CCD for higher resolution imaging

FEI Philips CM100

FEI Philips CM10

  • Both equipped with AMT (2k x 2k) CCD

Other Equipment

Unchained Labs pUNk dynamic light scattering (DLS) protein sizing system

Our new DLS system.

Light Microscope

Nikon 80i

Instec cryo-stage for use in cryo Correlative Light Electron Microscopy (CLEM) as well as live-cell imaging prior to fixation.

Sample Preparation Equipment

Wohlwend High Pressure Freezer

Wohlwend Compact 02 High Pressure Freezer

Cryofixation tool capable of generating specimens for cryo-sectioning, freeze-substitution and freeze-etching.

Technai F20

Leica EM BAF060 Freeze-etch System

Bal-Tec BAF060 freeze-etch-shadowing system for generation of high resolution replicas for TEM.

FEI Vitrobot

FEI Vitrobot Mark IV

Manual plunge freeze device

Other Sample Preparation Equipment

    Automatic Freeze-Substitution Systems (AFS)

    Microtomes

    • Leica Ultracut UCT (plastic)
    • Leica UC6

    Electron Microscopy Sciences Carbon Evaporator

    EMITEC Glow Discharger

    Tissue Culture Facility

    Computer Work Stations

Last updated: 2017-07-07

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